Instrumentation

1.) Aurora 10 Blue and Aurora 10 Black BST W1005

2.) ImageStream BST E1005

3.) Aria-10 BST E1005A

4.) FORTESSA-15 BST E1514

5.) LSR-10 BST E1005

6.) LSR-15 BST E1514

7.) FORTESSA-7 South BST S756

8.) ARIA-15 BST E1514

9.) FORTESSA-12 BST E1211

10.) ARIA-10 Hood BST E1005A

11.) ATTUNE NxT BST W1005 *NEW*

AURORA-10 Blue and Aurora-10 Black BST W1005

The Auroras are equipped with 5 lasers - 488 (blue), 640 (red), 405 (violet), 561 (yellow-green) 355 (UV).

64 Fluorescence channels:  355nm = 16 channels; 405nm = 16 channels; 488nm = 14 channels;  640nm = 8 channels; 561nm = 10 channels.  Efficient spectrum capture for dyes emitting in the 360nm-900nm range.  No filter changes required for any fluorochromes.

The Aurora Black has a plate loader for 96 well plates.

Cytek web page: click here

Aurora tested fluorochromes:  click here

 

ImageStream-10

Lasers:  488nm 200mW - 561nm 200mW - 375nm 70mW - 405nm 175mW - 642nm 150mW - 785nm Dark Field Laser

MultiMag 60X,40X,20X

Extended Depth of Field (EDF) 

Auto-Sampler (96 well plates)

12 Imaging Channels

For Configuration, click here.

For Fluorofinder panel design, click here.

 
Detector name Band (nm) Fluorochromes
Channel 1 435-480 475/45 DAPI, BV421, PacBlue
Channel 2 480-560 428/65 BV510, PacOrange -- FITC, GFP
Channel 3 569-595 528/65 QD565 -- Cy3, DSRed -- PE

Channel 4

 595-642 610/30 QD625 -- PE-TXRed, RFP -- mCherry
Channel 5 642-745 702/85 QD705, BV711 -- PerCP -- PeCy5, DRAQ5
Channel 6 745-780 762/35 QD800,BV786 -- PeCy7 --Pe-AF750
Channel 7 435-505 457/45 DAPI, BV 421
Channel 8 505-570 537/65 BV510, QD525
Channel 9 570-595 582/25 QD565,QD585 -- Bright Field
Channel10 595-642 610/30 QD625,BV605
Channel 11 642-745 702/85 QD705,BV711 -- APC, AF700
Channel 12 745-780 762/35 BV786 -- APC-Cy7 -- AF750

ARIA-10 BST E1005A

The Aria is equipped with 3 lasers 488 (blue), 633 (red), 405 (violet), for a total of 10 fluorescent parameters running on Diva 8/Windows 7. For configuration, click here. For Fluorofinder panel design, click here.

Detector name LP Mirror BP Filter Fluorochromes
488 A Laser 735 780/60 PE-CY7
488 B Laser 635 670/14 PE-CY5, 7AAD PerCP
488 B Laser 685 695/40 PE-CY5.5, PerCP-CY5.5 E
488 C Laser 600 610/20 PE-TxRED, E CD
488 D Laser 550 575/26 PE
488 E Laser 505 530/50 FITC, GFP
488 F Laser --- 488/10 SSC
633 A Laser 785 780/60 APC-Cy7
633 B Laser 735 720/20 Alexa 700
633 C Laser --- 670/30 APC
405 A Laser 505 525/50 V500
405 B Laser --- 450/50 Pacific Blue, Alexa405 DAPI

FORTESSA-15 BST E1514

The FORTESSA is equipped with 5 lasers 488 (blue), 640 (red), 405 (violet), 532 (Green), 355 (UV) for a total of 16 fluorescent parameters, running on Diva 8/Windows 7. Lasers: 405 100mW Trigon, 488 50mW Octagon, 532 150mW Octagon, 640 100mW Trigon, 355 20mW Trigon. For configuration, click here. For Fluorofinder panel design, click here.

Detector name LP Mirror BP Filter Fluorochromes
532 Laser A 750 780/60 PE-CY7
532 Laser B 670 695/40 PE-CY5.5
532 Laser C 635 670/30 PE-Cy5
532 Laser D 600 610/20 PE-TxRED,ECD
532 Laser E --- 575/26 PE
640 Laser A 785 780/60 APC-Cy7
640 Laser B 735 720/20 Alexa 700
640 Laser C --- 660/20 APC
405 Laser A 595 610/20 BV605
405 Laser B 505 525/50 V500
405 Laser C --- 450/50 Pacific Blue
488 Laser A 685 710/50 PerCP Cy5.5
488 Laser B 655 670/30 PerCP
488 Laser C 505 530/30 (515/20) FITC (GFP)
488 Laser D --- 488/10 SSC
355 Laser A 690 740/35 BUV 737
  505 530/30 Hoechst
355 Laser B --- 379/28 BUV 395
    450/50 DAPI, Alexa 350

LSR-10 BST E1005

The LSR II is equipped with 5 lasers running  on Diva 8/Windows 7. Lasers: 355 20mW Trigon, 405 50mW Trigon, 488 20mW Trigon, 532 150mW Octagon, 640 100mW Trigon.  15 parameters. For configuration, click here. For Fluorofinder panel design, click here.

Detector name LP Mirror

BP Filter

 Fluorochromes
488 Laser A 635 695/40 PerCP/PerCP-Cy5.5
488 Laser B 505 525/50 FITC, GFP
488 Laser C --- 488/10 SSC
532 Laser A 705 780/60 PE-Cy7
532 Laser B 685 710/50 PE-C5.5
532 Laser C 635 670/30 PE-Cy5
532 Laser D 595 610/20 PETxRED/PI
532 Laser E --- 575/20 PE
640 Laser A 755 780/60 APC-Cy7
640 Laser B 700 720/20 Alexa 700
640 Laser C --- 660/20 APC
405 Laser A 595 610/20 BV605
405 Laser B 505 525/50 V500, BV510, Alexa 430
405 Laser C --- 450/50 Pacific Blue, Alexa 405, BV421,V450
355 Laser A 690 740/35 BUV 737
  505 530/30 Indo 1
    670LP Red Side pop.
355 Laser B --- 379/28 BUV 395

 

   405/20 Indo-1 High
   

450/50

 

 DAPI, Alexa 350, Indo Violet

LSR-15 BST E1514

The LSR II is equipped with 5 lasers running on Diva 8/Windows 7.   Lasers:  355 60mW Trigon, 405 50mW Trigon, 488 20mW Trigon, 532 150mW Octagon, 633 40mW Trigon. 15 Parameters For configuration, click here. For Fluorofinder panel design, click here.

Detector name LP Mirror BP Filter Fluorochromes
488 Laser A 635 695/40

PerCP/PerCP-Cy5.5
488 Laser B 505 515/20 FITC/GFP
488 Laser C --- 488/10 SSC
532 Laser A 755 780/60 PE-Cy7
532 Laser B 685 710/50

PE-Cy5.5
532 Laser C 635 670/30

PE-Cy5
532 Laser D 595 610/20 PETxRED/PI
532 Laser E --- 575/20

PE
640 Laser A 755 780/60 APC-Cy7, APC-H7
640 Laser B 700  720/20 Alexa 700
640 Laser C --- 660/20 APC
405 Laser A 595 610/20 BV605
405 Laser B 505 525/50 V500, BV510
405 Laser C --- 450/50 Pacific Blue, BV421,V450
355 Laser A 690 740/35 BUV 737
  505 530/30 Indo-1, Hoechst
355 Laser B --- 379/28 BUV 395
    405/20 Indo-1 High
    450/50 DAPI, Indo-1 low

 

FORTESSA-7 South BST S756

The FORTESSA is equipped with 4 lasers 488 (blue), 640 (red), 405 (violet), 561 (Green),  for a total of 13 fluorescent parameters, running on Diva 8/Windows 7. Lasers: 405 100mW Trigon, 488 20mW Trigon, 561 100mW Octagon, 640 40mW Trigon. For configuration, click here. For Fluorofinder panel design, click here.

Detector Name LP Mirror BP Filter Fluorochromes
561 Laser A 750 780/60 PE-CY7
561 Laser B 670 695/40 PE-CY5.5
561 Laser C 635 670/30 PE-Cy5
561 Laser D 600 610/20 PE-TxRED,ECD
561 Laser E --- 582/15 PE
640 Laser A 750 780/60 APC-Cy7
640 Laser B 685 720/20 Alexa 700
640 Laser C --- 670/30 APC
405 Laser A 600 610/20 BV605
405 Laser B 505 525/50 V500
405 Laser C --- 450/50 Pacific Blue
488 Laser A 685 710/50 PerCP Cy5.5
       
488 Laser B 505 530/30 (515/20) FITC (GFP)
488 Laser C --- 488/10 SSC
     

ARIA-15 BST E1514

The Aria is equipped with 5 lasers running Diva 8 on Windows 7.  Lasers: 355 20mW Trigon, 405 50mW Trigon, 488 100mW Trigon, 532 150mW Octagon, 635 30mW Trigon. 15 Paramaters. For configuration, click here. For Fluorofinder panel design, click here.

 

Detector name LP Mirror BP Filter Fluorochromes
488 Laser A 685 695/40 PerCP/PerCP-Cy5.5
488 Laser B 505 520/15 FITC, GFP
488 Laser C --- 488/10 SSC
532 Laser A 740 780/40 PE-Cy7
532 Laser B 690 710/50 PE-C5.5
532 Laser C 640 660/40 PE-Cy5
532 Laser D 600 610/20 PETxRED/PI
532 Laser E --- 575/26 PE
640 Laser A 735 780/60 APC-Cy7
640 Laser B 710 730/45 Alexa 700
640 Laser C --- 660/20 APC
405 Laser A 600 605/40 BV 610
405 Laser B 505 525/50 V500, BV510, Alexa 430
405 Laser C --- 450/50 Pacific Blue, Alexa 405, BV421,V450
355 Laser A 505 530/30 Indo Blue
  690 740/35 BUV
    670LP Red Side pop.
355 Laser B --- 379/28 BUV 395
    405/20 Indo-1 High
    450/40 DAPI, Alexa 350, Indo Violet
 

 

Fortessa-12 BST E1211

The VMI Fortessa is equipped with 4 lasers running Diva 8/Windows 7.  Lasers: 355 20mW Trigon, 405 100mW Octagon, 488 100mW Octagon,  640 100mW Trigon. 13 Parameters.  For configuration, click here. For Fluorofinder panel design, click here.

Detector name LP Mirror BP Filter Fluorochromes
488 Laser A 750 780/60 PE-Cy7
488 Laser B 685 710/50 PerCP-Cy5.5
488 Laser C 600 610/20 PE-TxRED
488 Laser D 550 575/25 PE
488 Laser E 505 530/30 FITC
488 Laser F --- 488/10 SSC
       
640 Laser A 750 780/60 APC-Cy7
640 Laser B 690 730/45 Alexa 700
640 Laser C --- 670/30 APC
405 Laser A 630 660/20 Qdot655
405 Laser B 595 605/40

Qdot605
405 Laser C 505 525/50

AmCyan
405 Laser D --- 450/50 Pacific Blue
    670LP Red Side pop.
355 Laser A --- 379/28

BUV395
    405/20 Indo-1 High
    450/50 DAPI

Aria-10 Hood BST E1005A

The Rheum Aria is equipped with 5 lasers running Diva 8/Windows 7. Lasers:  355 60mW Trigon, 405 100mW Trigon, 488 100mW Trigon, 532 100mW Octagon, 640 100mW Trigon.  15 Paramaters. For configuration, click here. For Fluorofinder panel design, click here.

 

Detector name LP Mirror BP Filter Fluorochromes
532 Laser A 750 780/60 PE-Cy7
532 Laser B 690 710/50 Pe-Cy5.5
532 Laser C 595 670/40 Pe-Cy5
532 Laser D 550 610/20 PE-TxRED
532 Laser E 505 575/25 PE
488 Laser C 690 695/40 PerCP Cy5.5
488 Laser B 505 515/20 FITC
488 Laser A 488   SSC
640 Laser A 750 780/60 APC-Cy7
640 Laser B 690 730/45 Alexa 700
640 Laser C --- 670/30 APC
405 Laser A 570 585/42 BV605
405 Laser B 505 525/50

V500,BV510
405 Laser C --- 450/50

PacBlue, V450,BV421
355 Laser A 690 740/35 BUV737
355 Laser B   379/28 BUV393

Attune NxT BST W1005

The Attune NxT is equipped with 4 lasers 488 (blue), 640 (red), 405 (violet), 561 (Green),  for a total of 14 fluorescent parameters, running Windows 7. Lasers: 405 100mW Trigon, 488 100mW Trigon, 561 140mW Octagon, 640 140mW Trigon. For configuration, click here. For Fluorofinder panel design, click here.

Detector Name LP Mirror BP Filter Fluorochromes
532 Laser A 740 780/60 PE-CY7
532 Laser B 650 695/40 PE-CY5.5
532 Laser C 600 620/15 PE-TxRED, ECD
532 Laser D 550 575/36 PE
       
640 Laser A 740 780/60 APC-Cy7
640 Laser B 690 720/30 Alexa 700
640 Laser C 654 670/14 APC
405 Laser A 650 710/50 BV711
405 Laser B 555 603/48 BV605
405 Laser C 495 512/25 BV510
405 Laser D 417 440/50 BV421
488 Laser A 650 695/40 PerCP, PerCP-Cy5.5
488 Laser B 555 590/40 PE
488 Laser C 503 525/50 FITC