Instrumentation

Please click on the instrument name for more information about its current configuration.  You can schedule time on any instrument you have been trained to use via ScheduleBook.  To schedule a sort on any of the sorters listed below, please use the Sort Request Form.  Please refer to Core policies on Scheduling and Cancelation

When planning new experiments, we recommend using the panel builder app at FluoroFinder (free registration for Pitt users).

If you experience any issues with a machine after hours or on a weekend, please use the Problem Log to report it, and we will resolve the issue as soon as we can.

Instrument Name   Type Location Name in FluoroFinder

Aria Assembly Hood

(was Aria 10 Hood)

Traditional Flow Sorter Ford - G536 Aria Assembly G536
Attune Traditional Flow Analyzer Ford - G530 Attune NxT Assembly G530
Aurora Blue Spectral Flow Analyzer Ford - G530 Aurora Blue Assembly G530
Aurora Pink Spectral Flow Analyzer Ford - G530 (same config as Aurora Blue)

Fortessa Assembly

(was Fortessa 15)

Traditional Flow Analyzer Ford - G530 Fortessa Assembly G530
ImageStream Imaging Flow Analyzer Ford - G536 ImageStream Mark II Assembly G536

LSR Assembly

(was LSR 10)

Traditional Flow Analzer Ford - G530 LSR II Assembly G530
ZellScanner Chip Cytometer Ford - G536 N/A
       

Aria-15 3 Laser

(was Aria 10)

Traditional Flow Sorter BST - E1514 Aria 3 Laser | BST E1514

Aria-15 Hood 5 Laser

(was Aria 15 Hood)

Traditional Flow Sorter BST - E1514 Aria 5 Laser BST E1514
Aurora Black Spectral Flow Analyzer BST - E1514 Aurora Black BST E1514
Fortessa 7S (Hood) Traditional Flow Analyzer BST - S756 Fortessa BST S756
Fortessa 12 Traditional Flow Analyzer BST - E1211 Fortessa BST E1211
LSR 15 Traditional Flow Analyzer BST - E1514 LSR II BST E1514
       

 

AURORA

The Auroras are equipped with 5 lasers - 488 (blue), 640 (red), 405 (violet), 561 (yellow-green) 355 (UV).

64 Fluorescence channels:  355nm = 16 channels; 405nm = 16 channels; 488nm = 14 channels;  640nm = 8 channels; 561nm = 10 channels.  Efficient spectrum capture for dyes emitting in the 360nm-900nm range.  No filter changes required for any fluorochromes.

Aurora Black has a plate loader for 96 well plates.

Aurora Pink hase a plate loader for 96 well plates and a 40 tube holder.

Cytek web page: click here

Aurora tested fluorochromes:  click here

Aurora filter ranges:  click here

IMAGESTREAM

Lasers:  488nm 200mW - 561nm 200mW - 375nm 70mW - 405nm 175mW - 642nm 150mW - 785nm Dark Field Laser

MultiMag 60X,40X,20X

Extended Depth of Field (EDF) 

Auto-Sampler (96 well plates)

12 Imaging Channels

For Configuration, click here.

Detector name Band (nm) Fluorochromes
Channel 1 435-480 475/45 DAPI, BV421, PacBlue
Channel 2 480-560 428/65 BV510, PacOrange -- FITC, GFP
Channel 3 569-595 528/65 QD565 -- Cy3, DSRed -- PE

Channel 4

595-642 610/30 QD625 -- PE-TXRed, RFP -- mCherry
Channel 5 642-745 702/85 QD705, BV711 -- PerCP -- PeCy5, DRAQ5
Channel 6 745-780 762/35 QD800,BV786 -- PeCy7 --Pe-AF750
Channel 7 435-505 457/45 DAPI, BV 421
Channel 8 505-570 537/65 BV510, QD525
Channel 9 570-595 582/25 QD565,QD585 -- Bright Field
Channel10 595-642 610/30 QD625,BV605
Channel 11 642-745 702/85 QD705,BV711 -- APC, AF700
Channel 12 745-780 762/35 BV786 -- APC-Cy7 -- AF750

ARIA-15 3 LASER (was Aria 10)

The Aria is equipped with 3 lasers 488 (blue), 633 (red), 405 (violet), for a total of 10 fluorescent parameters running on Diva 8/Windows 7. For configuration, click here. For Fluorofinder panel design, click here.

Detector name LP Mirror BP Filter Fluorochromes
488 A Laser 735 780/60 PE-CY7
488 B Laser 635 670/14 PE-CY5, 7AAD PerCP
488 B Laser 685 695/40 PE-CY5.5, PerCP-CY5.5 E
488 C Laser 600 610/20 PE-TxRED, E CD
488 D Laser 550 575/26 PE
488 E Laser 505 530/50 FITC, GFP
488 F Laser --- 488/10 SSC
633 A Laser 785 780/60 APC-Cy7
633 B Laser 735 720/20 Alexa 700
633 C Laser --- 670/30 APC
405 A Laser 505 525/50 V500
405 B Laser --- 450/50 Pacific Blue, Alexa405 DAPI

FORTESSA ASSEMBLY (was Fortessa 15)

The FORTESSA is equipped with 5 lasers 488 (blue), 640 (red), 405 (violet), 532 (Green), 355 (UV) for a total of 16 fluorescent parameters, running on Diva 8/Windows 7. Lasers: 405 100mW Trigon, 488 50mW Octagon, 532 150mW Octagon, 640 100mW Trigon, 355 20mW Trigon. For configuration, click here. For Fluorofinder panel design, click here.

Detector name LP Mirror BP Filter Fluorochromes
532 Laser A 750 780/60 PE-CY7
532 Laser B 670 695/40 PE-CY5.5
532 Laser C 635 670/30 PE-Cy5
532 Laser D 600 610/20 PE-TxRED,ECD
532 Laser E --- 575/26 PE
640 Laser A 785 780/60 APC-Cy7
640 Laser B 735 720/20 Alexa 700
640 Laser C --- 660/20 APC
405 Laser A 595 610/20 BV605
405 Laser B 505 525/50 V500
405 Laser C --- 450/50 Pacific Blue
488 Laser A 685 710/50 PerCP Cy5.5
488 Laser B 655 670/30 PerCP
488 Laser C 505 530/30 (515/20) FITC (GFP)
488 Laser D --- 488/10 SSC
355 Laser A 690 740/35 BUV 737
  505 530/30 Hoechst
355 Laser B --- 379/28 BUV 395
    450/50 DAPI, Alexa 350

LSR ASSEMBLY (was LSR 10)

The LSR II is equipped with 5 lasers running  on Diva 8/Windows 7. Lasers: 355 20mW Trigon (UV), 405 50mW Trigon (violet), 488 20mW Trigon (blue), 532 150mW Octagon (green), 640 100mW Trigon (red).  15 parameters. For configuration, click here.  Fluorofinder panel design, click here.

Detector name LP Mirror

BP Filter

Fluorochromes
488 Laser A 635 695/40 PerCP/PerCP-Cy5.5
488 Laser B 505 525/50 FITC, GFP
488 Laser C --- 488/10 SSC
532 Laser A 705 780/60 PE-Cy7
532 Laser B 685 710/50 PE-C5.5
532 Laser C 635 670/30 PE-Cy5
532 Laser D 595 610/20 PETxRED/PI
532 Laser E --- 575/20 PE
640 Laser A 755 780/60 APC-Cy7
640 Laser B 700 720/20 Alexa 700
640 Laser C --- 660/20 APC
405 Laser A 595 610/20 BV605
405 Laser B 505 525/50 V500, BV510, Alexa 430
405 Laser C --- 450/50 Pacific Blue, Alexa 405, BV421,V450
355 Laser A 690 740/35 BUV 737
  505 530/30 Indo 1
    670LP Red Side pop.
355 Laser B --- 379/28 BUV 395

 

  405/20 Indo-1 High
   

450/50

 

DAPI, Alexa 350, Indo Violet

LSR-15

The LSR II is equipped with 5 lasers running on Diva 8/Windows 7.   Lasers:  355 60mW Trigon (UV), 405 50mW Trigon (violet), 488 20mW Trigon (blue), 532 150mW Octagon (green), 633 40mW Trigon (red). 15 Parameters For configuration, click here.  For Fluorofinder panel design, click here.

Detector name LP Mirror BP Filter Fluorochromes
488 Laser A 635 695/40

PerCP/PerCP-Cy5.5

488 Laser B 505 515/20 FITC/GFP
488 Laser C --- 488/10 SSC
532 Laser A 755 780/60 PE-Cy7
532 Laser B 685 710/50

PE-Cy5.5

532 Laser C 635 670/30

PE-Cy5

532 Laser D 595 610/20 PETxRED/PI
532 Laser E --- 575/20

PE

640 Laser A 755 780/60 APC-Cy7, APC-H7
640 Laser B 700  720/20 Alexa 700
640 Laser C --- 660/20 APC
405 Laser A 595 610/20 BV605
405 Laser B 505 525/50 V500, BV510
405 Laser C --- 450/50 Pacific Blue, BV421,V450
355 Laser A 690 740/35 BUV 737
  505 530/30 Indo-1, Hoechst
355 Laser B --- 379/28 BUV 395
    405/20 Indo-1 High
    450/50 DAPI, Indo-1 low

 

FORTESSA-7S 

The FORTESSA is equipped with 4 lasers 488 (blue), 640 (red), 405 (violet), 561 (Green),  for a total of 13 fluorescent parameters, running on Diva 8/Windows 7. Lasers: 405 100mW Trigon, 488 20mW Trigon, 561 100mW Octagon, 640 40mW Trigon. For configuration, click hereFor Fluorofinder panel design, click here.

Detector Name LP Mirror BP Filter Fluorochromes
561 Laser A 750 780/60 PE-CY7
561 Laser B 670 695/40 PE-CY5.5
561 Laser C 635 670/30 PE-Cy5
561 Laser D 600 610/20 PE-TxRED,ECD
561 Laser E --- 582/15 PE
640 Laser A 750 780/60 APC-Cy7
640 Laser B 685 720/20 Alexa 700
640 Laser C --- 670/30 APC
405 Laser A 600 610/20 BV605
405 Laser B 505 525/50 V500
405 Laser C --- 450/50 Pacific Blue
488 Laser A 685 710/50 PerCP Cy5.5
       
488 Laser B 505 530/30 (515/20) FITC (GFP)
488 Laser C --- 488/10 SSC
     

ARIA-15 HOOD 5 LASER (was Aria 15 Hood)

The Aria is equipped with 5 lasers running Diva 8 on Windows 7.  Lasers: 355 20mW Trigon, 405 50mW Trigon, 488 100mW Trigon, 532 150mW Octagon, 635 30mW Trigon. 15 Paramaters. For configuration, click here. For Fluorofinder panel design, click here.

 

Detector name LP Mirror BP Filter Fluorochromes
488 Laser A 685 695/40 PerCP/PerCP-Cy5.5
488 Laser B 505 520/15 FITC, GFP
488 Laser C --- 488/10 SSC
532 Laser A 740 780/40 PE-Cy7
532 Laser B 690 710/50 PE-C5.5
532 Laser C 640 660/40 PE-Cy5
532 Laser D 600 610/20 PETxRED/PI
532 Laser E --- 575/26 PE
640 Laser A 735 780/60 APC-Cy7
640 Laser B 710 730/45 Alexa 700
640 Laser C --- 660/20 APC
405 Laser A 600 605/40 BV 610
405 Laser B 505 525/50 V500, BV510, Alexa 430
405 Laser C --- 450/50 Pacific Blue, Alexa 405, BV421,V450
355 Laser A 505 530/30 Indo Blue
  690 740/35 BUV
    670LP Red Side pop.
355 Laser B --- 379/28 BUV 395
    405/20 Indo-1 High
    450/40 DAPI, Alexa 350, Indo Violet
 

Fortessa-12 

The VMI Fortessa is equipped with 4 lasers running Diva 8/Windows 7.  Lasers: 355 20mW Trigon (UV), 405 100mW Octagon (violet), 488 100mW Octagon (blue),  640 100mW Trigon (red). 15 Parameters.  For configuration, click here.  For Fluorofinder panel design, click here.

Detector name LP Mirror BP Filter Fluorochromes
488 Laser A 750 780/60 PE-Cy7
488 Laser B 685 710/50 PerCP-Cy5.5
488 Laser C 600 610/20 PE-TxRED
488 Laser D 550 575/25 PE
488 Laser E 505 530/30 FITC
488 Laser F --- 488/10 SSC
       
640 Laser A 750 780/60 APC-Cy7
640 Laser B 690 730/45 Alexa 700
640 Laser C --- 670/30 APC
405 Laser A 630 660/20 Qdot655
405 Laser B 595 605/40

Qdot605

405 Laser C 505 525/50

AmCyan

405 Laser D --- 450/50 Pacific Blue
    670LP Red Side pop.
355 Laser A 690 740/37

BUV737

355 Laser B   379/28 BUV395
    450/50 DAPI

ARIA ASSEMBLY HOOD (was Aria 10 Hood)

The Rheum Aria is equipped with 5 lasers running Diva 8/Windows 7. Lasers:  355 60mW Trigon (UV), 405 100mW Trigon (Violet), 488 100mW Trigon (Blue), 532 100mW Octagon (Green), 640 100mW Trigon (Red).  15 Paramaters. For configuration, click here. For Fluorofinder panel design, click here.

 

Detector name LP Mirror BP Filter Fluorochromes
532 Laser A 750 780/60 PE-Cy7
532 Laser B 690 710/50 Pe-Cy5.5
532 Laser C 595 670/40 Pe-Cy5
532 Laser D 550 610/20 PE-TxRED
532 Laser E 505 575/25 PE
488 Laser C 690 695/40 PerCP Cy5.5
488 Laser B 505 515/20 FITC
488 Laser A 488   SSC
640 Laser A 750 780/60 APC-Cy7
640 Laser B 690 730/45 Alexa 700
640 Laser C --- 670/30 APC
405 Laser A 570 585/42 BV605
405 Laser B 505 525/50

V500,BV510

405 Laser C --- 450/50

PacBlue, V450,BV421

355 Laser A 690 740/35 BUV737
355 Laser B   379/28 BUV393

ATTUNE

The Attune NxT is equipped with 4 lasers 488 (blue), 640 (red), 405 (violet), 561 (Green),  for a total of 14 fluorescent parameters, running Windows 7. Lasers: 405 100mW Trigon, 488 100mW Trigon, 561 140mW Octagon, 640 140mW Trigon.  For Fluorofinder panel design, click here.

Detector Name LP Mirror BP Filter Fluorochromes
532 Laser A 740 780/60 PE-CY7
532 Laser B 650 695/40 PE-CY5.5
532 Laser C 600 620/15 PE-TxRED, ECD
532 Laser D 550 575/36 PE
       
640 Laser A 740 780/60 APC-Cy7
640 Laser B 690 720/30 Alexa 700
640 Laser C 654 670/14 APC
405 Laser A 650 710/50 BV711
405 Laser B 555 603/48 BV605
405 Laser C 495 512/25 BV510
405 Laser D 417 440/50 BV421
488 Laser A 650 695/40 PerCP, PerCP-Cy5.5
488 Laser B 555 590/40 PE
488 Laser C 503 525/50 FITC

CANOPY ZELLSCANNER ONE

Canopy's ZellScanner One is a fully intergrated automated imaging system that enables quantitaitve measure of high-plex protein biomarker assays across a range of sample types.  Termed "Chip Cytometry", single cell and whole tissue samples can be loaded onto a chip - the latter via section-on-coverslip attachment.  The enlosed chamber acts as a biorepository, providing antibody staining and washing via stain/wash/photobleach cycles, and then stored with tissue preservation for up to two years.  This non-destructive analysis allows for multiple interrogations of the same sample.

Filter Sets
ZellScanner Filter Set Name Excitation Emission
FS395 BP 365/2 nm BP 392/23 nm
FS421 BP 390/40 BP 460/50
FS488 BP 470/40 BP 525/50
FS560 BP 550/25 BP 605/70
FSPerCP BP 435/40 BP 710/75
ALL (Bleach) LP 364