Carolyn Coyne, PhD
Campus: 4401 Penn Avenue
Office: 9116 Rangos Research Center
Pittsburgh, PA 15224
- BS in Biochemistry, Florida State University
- PhD in Pharmacology, University of North Carolina, Chapel Hill
- Post-doctoral, Department of Microbiology, University of Pennsylvania
- Associate Professor, Pediatrics
- Associate Professor, Imunology
The research in my lab combines important aspects of virology, immunology, and cell biology to dissect various aspects of host-virus interactions, with a particular focus on polarized cell types. In particular, we focus on—(1) on the mechanisms by which enteroviruses have evolved to successfully circumnavigate the barriers presented by polarized cells to facilitate virus entry, replication, and spread, (2) on the mechanisms utilized by enteroviruses to evade and/or suppress innate immune signaling, (3) on the identification of novel regulators and components of the innate immune system, and (4) on the role of placental-specific microRNAs in antiviral signaling and the induction of autophagy at the maternal-fetal interface.
Delorme-Axford E, Sadovsky Y, Coyne C. B. Lipid raft- and SRC family kinase-dependent entry of coxsackievirus B into human placental trophoblasts. J Virol. 87: 8569-8581.
Delorme-Axford E, Donker R. B, Mouillet J. F, Chu T, Bayer A, Ouyang Y, Wang T, Stolz D. B, Sarkar S. N, Morelli A. E, Sadovsky Y, Coyne C. B. Human placental trophoblasts confer viral resistance to recipient cells. Proc Natl Acad Sci USA. 110: 12048-12053.
Bozym R. A, Delorme-Axford E, Harris K, Morosky S, Ikizler M, Dermody T. S, Sarkar S. N, and Coyne C. B. Focal adhesion kinase is a component of RIG-I-like receptor antiviral signaling. Cell Host Microbe. 11: 153-166.
Mukherjee A, Morosky S. A, Delorme-Axford E, Dybdahl-Sissoko N, Oberste M. S, Wang T, and Coyne C. B. The coxsackievirus B 3C protease cleaves MAVS and TRIF to attenuate host type I interferon and apoptotic signaling. PLoS Pathog. 7: e1001311.
Coyne C. B, Bozym R, Morosky S. A, Hanna S. L, Mukherjee A, Tudor M, Kim K. S, Cherry S. Comparative RNAi screening reveals host factors involved in enterovirus infection of polarized endothelial monolayers. Cell Host Microbe. 9: 70-82.
Bozym R. A, Patel K, White C, Cheung K. H, Bergelson J. M, Morosky S. A, and Coyne C. B. Calcium signals and calpain-dependent necrosis are essential for release of coxsackievirus B from polarized intestinal epithelial cells. Mol Biol Cell. 22: 3010-3021.
Bozym R. A, Morosky S. A, Kim K. S, Cherry S, and Coyne C. B. Release of intracellular calcium stores facilitates coxsackievirus entry into polarized endothelial cells. PLoS Pathog. 6: e1001135.