Department Resources

Flow Cytometry

The Unified Flow Core provides state-of-the-art flow cytometry and cell sorting services to the entire University of Pittsburgh research community as well as researchers at neighboring institutions. A skilled staff provides help with instrument setup, data analysis, and consultation for experiment design. Training is available to enable investigators and their staff to run the analytical cytometers themselves.

Transgenics and Gene Targeting

The TGT (Transgenic and Gene Targeting) and Innovative Technologies Development (ITD) Cores are a shared resource designed to produce genetically engineered mice for investigators of University of Pittsburgh. Our laboratory provides a broad, comprehensive range of services to investigators.

ITD Core

For information about the ITD core services, or to initiate a CRISPR project, visit our page, or contact Dr. Sebastien Gingras, ITD Core Director, at sgingras@pitt.edu.

TGT Core

For information about the TGT core services, or to initiate a project, visit our page at https://www.immunology.pitt.edu/transgenic-and-gene-targeting-tgt-core, or contact Dr. Chunming Bi, Core director, at chunming@pitt.edu or Binfeng Lu, Faculty advisor, at binfeng@pitt.edu

Epoch Microplate Spectrophotometer

Epoch™ has a monochromator-base UV-Vis wavelength selection. Wavelength range from 200 nm to 999 nm and 6- to 384- microplate reading capability, controlled with the Gen5 Data Analysis software interface. In addition, micro-volume quantification is fast and easy with the Take3 Micro-Volume plates. Pre-programmed nucleic acid and protein protocols in Gen5 make quantification fast and easy. For more information, please contact Pamela Beatty, 412-383-5287. 

BioRad Fluorescence/Chemiluminescence Imager

ChemiDoc Imagers offer best-in-class performance with ease of use for visible light (RGB) and far red/near infrared (FR/NIR) fluorescence and chemiluminescence detection and all general gel documentation applications. Stain-free imaging enables immediate visualization of proteins without gel staining and instant verification of protein transfer to blots. Features of the ChemiDoc MP include Chemiluminescence sensitivity matching X-ray film and multiplex fluorescence defection. For more information, please contact Rachel Gottschalk