The Unified Flow Core provides state-of-the-art flow cytometry and cell sorting services to the entire University of Pittsburgh research community as well as researchers at neighboring institutions. A skilled staff provides help with instrument setup, data analysis, and consultation for experiment design. Training is available to enable investigators and their staff to run the analytical cytometers themselves.
Transgenics and Gene Targeting
The TGT (Transgenic and Gene Targeting) and Innovative Technologies Development (ITD) Cores are a shared resource designed to produce genetically engineered mice for investigators of University of Pittsburgh. Our laboratory provides a broad, comprehensive range of services to investigators.
For information about the ITD core services, or to initiate a CRISPR project, visit our page at https://www.immunology.pitt.edu/core-facilities/itd-core, or contact Dr. Sebastien Gingras, ITD Core Director, at firstname.lastname@example.org.
For information about the TGT core services, or to initiate a project, visit our page at https://www.immunology.pitt.edu/transgenic-and-gene-targeting-tgt-core, or contact Dr. Chunming Bi, Core director, at email@example.com or Binfeng Lu, Faculty advisor, at firstname.lastname@example.org
This is a CTL Immunopot elispot reader using an Image Acquisition version 4.5 and Immunospot version 5.0 analysis software. It is capable of performing basic and double-color counting. There are no fees to use this instrument.
ProteinSimple FluorChem M Multifluor System
This system is used for digital imaging of western blots developed with ECL or fluoresent reagents in the red, green and blue spectrum. This equipment is available for departmental use only. No fees are applicable. For more information, please contact Larry Kane.
Epoch Microplate Spectrophotometer
Epoch™ has a monochromator-base UV-Vis wavelength selection. Wavelength range from 200 nm to 999 nm and 6- to 384- microplate reading capability, controlled with the Gen5 Data Analysis software interface. In addition, micro-volume quantification is fast and easy with the Take3 Micro-Volume plates. Pre-programmed nucleic acid and protein protocols in Gen5 make quantification fast and easy. For more information contact Pamela Beatty, 412-383-5287.