- Upcoming Seminar(s)
- Coming soon (Fall 2026):
- September: Seminar for Sorting with the Unified Flow Core
- October: Seminar for Spectral Flow Cytometry
- Be sure you're on our email list to receive information on upcoming seminars from the Unified Flow Core. Sign up under the "Request Services" tab in iLab.
- Please send requests for future seminar topics to flowcore@pitt.edu.
- Recordings and/or slides from past seminars can be found in the next section: "Past Unified Flow Core Seminars."
- Coming soon (Fall 2026):
- Past Unified Flow Core Seminars
- Introduction to Flow Cytometry
- View recording here (Panopto via Pitt).
- Presented by Nicole Brandon, M.S. on August 21, 2026.
- Combining Flow Cytometry and Microscopy: Applications of the ImageStream MkII
- presented by Nevil Abraham on July 21, 2026
- View recording here (Panopto via Pitt).
- FluoroFinder - Online Webinar - Tuesday, October 28
- Can view recording here (need to register for free):
- Training about how to use and make the most out of the FluoroFinder platform—an essential tool for designing fluorescent experiments.
- Cytobank - Machine Learning Algorithms - May 28, 2025
- Cytek - ImageStream Webinar - May 28, 2025
- Internalization, Co-localization, and Spot Counting with ML (free registration required)
- See ImageStream section below for more webinars.
- Note: The Unified Flow Core has IDEAS available for use on the Analysis Computer (just sign up in iLabs).
- Sony FP700 - February 5, 2025
- https://pitt.hosted.panopto.com/Panopto/Pages/Viewer.aspx?id=ca63c824-222f-4f76-8034-b27b013b81fd
- There is a brief period late in the seminar where the sound cuts out but the video continues. The sound returns after a few min.
- Getting Sorting Sorted - Nicole Brandon, M.S. - November 2024
- BD Seminar of S8 Imaging Spectral Sorter - August 2024
- Design Panels with Confidence using Cytek's New Intelligent Panel Design Algorithm (Dr. Paul Dascani, Cytek) - August 2024
- FluoroFinder Seminar - May 2024
- New Autofluorescence Explorer tool for spectral data on collected Cytek Auroras - Dr. Paul Dascani - November 2023
- Presentation - Note the chapter divide to skip to software demo section. And I edited the transcript for you, so you can turn that on if needed.
- Raw NxN Worksheet for Spectroflo (This worksheet is available on the Desktops of the various Auroras.) Download and open in Spectroflo to use it.
- Handy list of common fluorochromes and their peak fluorescence channels on 5-laser Cytek Auroras (as all of ours are).
- ImageStream (webinars might be limited to Pitt users only)
- Intro to Advanced Flow Cytometry - Dr. Florian Weisel - Fall 2021
- Conventional vs. Spectral Flow - Dr. Lisa Borghesi - Fall 2021
- Aurora Panels (requires Pitt login)
- Introduction to Flow Cytometry - Aarika MacIntyre - 2021
- Conventional Flow Cytometry
- Background on Conventional Flow:
- ThermoFisher: https://www.youtube.com/watch?v=sfWWxFBltpQ
- UChicago https://voices.uchicago.edu/ucflow/training-courses/ (download Flow Basics PPT from here) Although at first it seems to go over many of the same topics as the ThermoFisher video, it then goes deeper... introducing spectral flow cytometry, data analysis, and ways to clean up your experiments/data.
- Other Flow resources at ThermoFisher.
- Other Flow resources at UChicago
- BD Courses (many are free/online)
- Introduction to Flow Cytometry - Aarika MacIntyre - 2021
- Intro to Advanced Flow Cytometry - Florian Weisel - Fall 2021
- LSR II and Fortessa Experiment Guide
- How to run experiments in Diva. (handout)
- LSR/Fortessa Course at UChicago
- Scroll down for video on how to use Diva, how to fill sheath, and troubleshooting guides.
- By University of Chicago's Cytology and Antibody Technology Facility.
- Troubleshooting and Advanced Declogging (LSR II & Fortessa)
- Requires Pitt Login. (turn down volume)
- Also see Troubleshooting Guide at UChicago (skip down to bottom 25% of page or ctrl-f (command F on Mac) and search for "Troubleshooting."
- FluoroFinder
- Get a free account as a member of Pitt. Very helpful for designing panels for specific machines. Can design spectral panels here as well.
- New feature as of 2025: "Cell Types." Can see which markers are commonly used to ID specific kinds of cells, with sample gating strategies, and literature references for more information.
- Introduced in webinar from Oct 2025 (free registration to view, do not need prior registration): https://attendee.gotowebinar.com/recording/6419504587313157212
- Background on Conventional Flow:
- Spectral Flow Cytometry
- Cytek (Aurora) Biosciences Resource Page
- Cytek Cloud - Good place for spectral panel design for Auroras (all of ours are 5 laser). Can even save an experiment template to save time setting up at the machine! Account is free.
- Aurora Training Course
- This is an EXCELLENT course on spectral flow cytometry by the University of Chicago's Cytology and Antibody Technology facility.
- Unified Flow Core Aurora Reference Guide
- Be sure to check out the AF Explorer Tool seminar from November 2023.
- Cytek (Aurora) Biosciences Resource Page
- ImageStream
- Webinars from Cytek (previously recorded, free registration required to view each):
- Basic Techniques (1/22/2025)
- Advanced Techniques (1/23/2025)
- Internalization, Co-localization, and Spot Counting with ML (5/28/2025)
- Sample Prep Guide
- Univ. Chicago's experiment setup training video: https://www.youtube.com/watch?v=otFE2CvWBaw
- (Also see Data Analysis section)
- (Also see Seminars section)
- Webinars from Cytek (previously recorded, free registration required to view each):
- Data Analysis Resources
- Flowjo
- Note: no account needed to view educational/reference materials. Flowjo can be used to analyze spectral and conventional flow data.
- Flowjo Courses
- Webinars Scroll down to "Recorded Webinars" if you aren't able to attend a live one.
- Flowjo Exchange Download various (free) plugins here. Especially recommended are: FlowAI, FlowSOM, ClusterExplorer, and check out ezDAFI. Recommended dimensional reduction plugins in addition to TSNE (which is now part of Flowjo), UMAP, FitSNE (faster TSNE)
- The support staff at Flowjo is very responsive and helpful. Feel free email them with questions about the software, plugin installation, or the plugins themselves: flowjo@bd.com
- Check out the next section as well for background on dimensional reduction. Most tools mentioned are now available as Flowjo plugins.
- ImageStream Data Analysis
- IDEAS - analysis software manual
- IDEAS Tutorial Series is available on Analysis Computer in Assembly G532
- Machine Learning Manual - IDEAS v6.3 has the machine learning module (this version is available on analysis computer in Assembly G532).
- Flowjo
- Flow Cytometry BootCamp Series - Lisa Borghesi (Fall 2021)
Other Links
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